Department of Basic Scince, College of Dentistry, University of Wasit /Kut, Iraq.
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(01), 647-652
Article DOI: 10.30574/wjarr.2022.16.1.1094
Received on 21 September 2022; revised on 22 October 2022; accepted on 25 October 2022
The goal of this research was to characterize ADI isolated from Lactobacillus sp. and test its anticancer activity in vitro. Because the arginine-degrading enzyme is a strong anticancer agent, it was isolated in two phases from a Lactobacillus sp. clinical isolate: 70% saturated ammonium sulphate precipitation followed by gel filtration chromatography (2x35cm). The most active fractions in enzyme activity (7.3 U/ ml), Purified enzyme had a special action activity of (48.6 U/mg) with nine folds of purgation and 61.4% enzyme recovery, The cytotoxic activity of purified arginine deiminase on PC3,A375 and WRL68 cells for 24 was examined.
Purified arginine deiminase inhibited the proliferation of cancer cell lines PC3 and A375, with IC50 values of 68.64 g/ml and 136.3 g/ml, respectively.
At a concentration of 6.25 ϻg/ml, 94.83% and 95.68% cell viability were observed of PC3 and A375 after treatment with purified arginine deiminase, respectively. However, cell viability reached to 42.52% and 48.77% using 400 ϻg/ml concentration purified arginine deiminase, respectively while ADI didn't show a significant effect on the viability of normal cell line.
Cytotoxic activity; Arginine deiminase; Lactobacillus sp; Anticancer activity
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Essmaa Hussein Gutef. Cytotoxic activity of arginine deiminase purified from Lactobacillus sp. World Journal of Advanced Research and Reviews, 2022, 16(1), 647-652. Article DOI: https://doi.org/10.30574/wjarr.2022.16.1.1094