Biosynthesis of silver nanoparticles mediated by entomopathogenic bacteria: Bacillus cereus VCRC 641 against Aedes aegypti, Anopheles stephensi and Culex quinquefasciatus

Manikandan Sivaprakasam, Jibi Lukose, Abhisubesh Vijayakumar, Hemaladkshmi Padmanaban, Aneha K, Sahadiya Mandodan, Kakhuangailiu Gangmei, Bhagyashree Bora, Mathivanan Ashokkumar, Vijayalakshmi Krishnamoorthy and Poopathi Subbiah *

Department of Health Research, Unit of Microbiology and Immunology, ICMR- Vector Control Research Centre, Ministry of Health and Family Welfare, Pondicherry- 605006, India.
 
Research Article
World Journal of Advanced Research and Reviews, 2022, 16(01), 311–319
Article DOI: 10.30574/wjarr.2022.16.1.1030
 
Publication history: 
Received on 08 September 2022; revised on 09 October 2022; accepted on 12 October 2022
 
Abstract: 
Mosquito-borne diseases are illnesses caused by the transmission of viruses, parasites, and protozoa to humans by mosquito vectors. Mosquitoes spread various diseases, including, dengue fever, malaria, chikungunya, West Nile fever, Zika and yellow fever. Bacterial DNA and cellular proteins were shown to help produce AgNPs, with bacterial DNA acting as a stabilising agent and cellular proteins acting as both a reducing and stabilising agent. As a result, the bacterial lysate, which contains a lot of DNA and proteins, could help in the creation of AgNPs that are larvicidal against mosquito larvae. Therefore, this work was aimed to study the larvicidal effect of synthesized AgNPs by Bacillus cereus VCRC 641 cell lysate. The present study demonstrates the larvicidal efficacy of silver nanoparticles (AgNPs) produced by Bacillus cereus VCRC 641 against the diseases-causing mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles stephensi. Silver nano particles were synthesized using Bacillus cereus to enhance its mosquitocidal efficacy. The level of toxicity in the AgNPs synthesized B. cereus was increased 117 fold in comparison with the cell lysate.
 
Keywords: 
AgNPs; Bacillus cereus; Aedes aegypti; Culex quinquefasciatus; Anopheles stephensi
 
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