1 Department of Pharmacology and Toxicology, Chukwuemeka Odumegwu Ojukwu University Igbariam campus Nigeria.
2 Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, P.M.B 5025 Awka,420110 Anambra state, Nigeria.
3 Department of Medical Laboratory Science and Biology, School of Nursing and Health Sciences, Neumann University, One Neumann Drive, Aston, PA, 19014. USA.
Research Article
World Journal of Advanced Research and Reviews, 2024, 24(01), 1356–1364
Received on 01 September 2024; revised on 10 October 2024; accepted on 12 October 2024
This research study was aimed at investigating the common folkloric use of Vitex doniana fruit extract for induction of sleep. Extraction of the fruit extract was carried out with 80 %v/v ethanol. Acute toxicity study and qualitative phytochemical screening of the extract were also done. Twenty-five adult mice divided into five groups (n=5) were used for both the anxiolytic study and the phenobarbital-sleeping time analysis tests. Groups I and II served as negative (distilled water 10 ml/kg) and positive (diazepam 1 mg/kg i.p.) controls, respectively. Groups III, IV and V received the extracts of 250, 500 and 1000 mg/kg p.o., respectively. The effect of extract on time spent in and number of entries into the open arms and closed arms when compared to control were noted. The effect on onset and duration of sleep -were also noted. The extract was found to be relatively safe. Secondary metabolites like tannins and flavonoids were present. There was a dose-related significant (p < 0.05) increase in time spent on the open arm and a decrease in time spent on closed arm when compared to the negative control. Dose-related significant (p < 0.05) decrease in time for sleep onset and increase in duration of sleep when compared to negative control were also observed. From the results of this study, the folkloric use of this fruit extract for the induction of sleep and relaxation seem to be justified.
Sedative; Anxiolytic; Sleeping-time; Vitex doniana
Preview Article PDF
Victoria Nonyelum Olli, Ernest Chima Orji and Jude Nnaemeka Okoyeh. Assessment of sedative and anxiolytic activities of fruit extract of Vitex doniana (black plum). World Journal of Advanced Research and Reviews, 2024, 24(1), 1356-1364. Article DOI: https://doi.org/10.30574/wjarr.2024.24.1.3117