1 Dental Health Science Postgraduate Program, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
2 Faculty of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia.
3 Faculty of Dentistry, Bhakti Wiyata Institute of Health Sciences, Kediri, East Java, Indonesia.
4 Undergraduate program, Faculty of Dentistry, Bhakti Wiyata Institute of Health Sciences, Kediri, East Java, Indonesia.
Research Article
World Journal of Advanced Research and Reviews, 2024, 24(01), 2185-2190
Received on 14 September 2024 ; revised on 21 October 2024; accepted on 23 October 2024
Based on the Global Burden of Disease Study in 2017, the prevalence of caries in each country reached 20% to more than 50%. Caries is formed due to the role of several factors including host, agent (microorganisms), substrate, and time. Bacteria involved in the origin of caries formation such as Lactobacillus group bacteria found in the oral cavity which can cause dental caries, one of which is Lactobacillus acidophilus. The use of natural cleansers is highly recommended to remove residual bacteria as an effort to prevent caries. Queen Pineapple (Ananas comosus (L.) Merr Var. Queen) is a fruit that contains very complex compounds that can act as anticarcinogenic and antibacterial. This study aims to determine the Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) against the growth of Lactobacillus acidophilus bacteria. This type of research was a laboratory experimental type with posttest only control group design. This study used the dilution method of the number of samples, namely concentrations of 0.781%, 1.56%, 3.125%, 6.25%, 12.5%, 25%, 50%, 100%, positive controls and negative controls. This study was conducted 3 times repetition. Manual calculation of the number of bacterial colonies that grow on nutrient agar media and expressed in colony forming units (CFU) which will determine the MBC and MIC. The Shapiro-Wilk test showed a p value> 0.05 at all concentrations and normally distributed data, but when tested for homogeneity the data was not homogeneous so that the non-parametric statistical test continued using the Kruskal Wallis test showing a value of p < 0.05. Conclusion: Colony count results showed that the concentration of 3.12% can inhibit the visible growth of Lactobacillus acidophilus bacteria which is stated as the Minimum Inhibitory Concentration (MIC) and can prevent growth after subculture to antibiotic-free medium at a concentration of 6.25% which is stated as the Minimum Bactericidal Concentration (MBC).
Queen Pineapple (Ananas comosus (L.) Merr Var. Queen) Peel Extract; Lactobacillus acidophilus; Minimum Inhibitory Concentration (MIC); Minimum Bactericidal Concentration (MBC)
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Elok Nafilah Fitri, Intan Nirwana, Asti Meizarini, Nur Dianawati, Basma Rosandi Prakosa, Fery Setiawan and Nur Fajar Vemellya. Antibacterial potential of queen pineapple (Ananas comosus (L.) Merr Var. Queen) peel extract on the growth of Lactobacillus acidophilus bacteria. World Journal of Advanced Research and Reviews, 2024, 24(1), 2185-2190. Article DOI: https://doi.org/10.30574/wjarr.2024.24.1.3248