Acute effect of the novel cadaver fixation and storage solution -Anatomical-biomechanical-microbiological study
1 Department of Anatomy, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye.
2 Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye.
3 Department of Biochemistry, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyonkarahisar, Türkiye.
Research Article
World Journal of Advanced Research and Reviews, 2024, 23(03), 1250–1260
Publication history:
Received on 26 July 2024; revised on 02 September 2024; accepted on 05 September 2024
Abstract:
Cadaver education is a golden standard in health sciences. It is aimed to purify the cadavers from microbes by subjecting them to chemicals and to be used for many years by terminating autolysis. As a result of literature searches, a solution consisting of a mixture of borax, nitrite-nitrate, glycerin, alcohol, and thyme oil was created. While seven sheep hearts were placed in this solution, seven were kept in 10% formaldehyde for two months for fixation and storage. At the end of the first ten days, it was observed that the discoloration of the heart in the solution was lower than the formalin fixation. In histological examinations, it was observed that there was no visual difference between the groups and the tissue could be examined at all magnifications. At the end of two months, insignificant microbial growth observed in the solution. While color changes were better than formaldehyde, texture profile analysis values were found to be closer to fresh heart. In histological examinations, in the solution group, although the tissue could be recognized at all magnifications, it was observed that cell nuclei were less stained at x100. It was concluded that this solution can be used mainly in short-term courses.
Keywords:
Boron; Cadaver; Fixation; Formaldehyde; Heart
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