A new tapeworm Stilesia jadhavae sp.nov.from the intestine of goat Capra hircus at Patoda tehsil of Beed district, MS, India

The present communication deals with the description of a new cestode Stilesia jadhavae sp.nov. from the intestine of goat Capra hircus at Patoda tehsil of Beed district. The examined parasites were long with scolex, immature, mature and gravid proglottids. The scolex of the worm is large, quadrangular, broad anteriorly, narrow posteriorly, distinctly marked off from the strobila; suckers are four, large in size, oval in shape, arranged in two pairs; neck short; mature segments small in size, broader than long having only one set of reproductive organs in each segment; testes 24 in number, medium, round in two lateral fields; cirrus pouch small in size, oval in shape; cirrus is thin coiled; vas deferens thin, small, coiled; ovary medium in size, bilobed; vagina thin coiled; ootype small in size, round in shape; genital pores are small in size, round in shape, regularly alternate; excretory canals narrow.


Introduction
The genus Stilesia was erected by Railliet in 1893, from Ovis aries in Europe, Asia and Africa, as Stilesia globipunctata. Later on S. hepatica was added to this genus by Wolffhugel in 1903 from sheep and goat in East Africa. Stilesia vittata was reported by Railliet in 1896 from Camelus dromedarius from Africa and India. Stilesia okapi is erected as a new species of this genus by Leiper in 1936 from Okapi in Africa, is regarded by Baer, 1950 as a variety of Stilesia globipunctata. The author agrees with Baer to regard Stilesia okapi as S. globipunctata.
Later on the following species are added to this genus by different workers in the world as-1) S. globipunctata (Rivolta, 1874) Railliet The present species of cestode parasite Stilesia jadhavae sp.nov.is one more addition in the previous record of taxonomy of cestode.

Material and methods
Sixteen specimens of the cestode parasites were collected from the intestine of goat Capra hircus. The collected parasites were long with thin musculature having numerous immature and mature segments. The worms were flattened, preserved in 4% formalin, stained with Harris haematoxylin, passed through various alcoholic grades, cleared in xylol, mounted in DPX and whole mount slides were taken for further anatomical studies. The drawings were made with the aid of Camera Lucida and all measurements in millimeters.

Description
The scolex of the worm is fairly large in size, quadrangular in shape, broader anteriorly, narrow posteriorly, marked off from the strobila and measures 0.

Results and discussion
The present communication deals with the description of new species Stilesia jadhavae sp.nov. After going through the literature the worm under discussion differs from the all known species.
 The present worm under discussion differs from Stilesia globipunctata, Railliet (1893) [1],in having the mature proglottid broader than long, with distinct segmentation; testes in two groups,4-7 on each side; vas deferens not closely coiled between cirrus pouch and outer wall of excretory canal; cirrus pouch small, pyriform, ventral to vagina; ovary globular, median; vagina dorsal to cirrus pouch, uterus globular, par uterine organs develops from uterus.  The present cestode differs from Stilesia vittata, Railliet (1896) [2], which is having mature segments broader than long, with indistinct segmentation; testes in two groups,5-9 on each side; vas deferens closely coiled; cirrus pouch elongated, cylindrical, cirrus armed; ovary rounded, situated between portal vessels; vagina posterior to cirrus pouch, uterus transverse, a large tube near the anterior margin; par uterine organs two in number, in each segment, big, developed from uterus and internal to the excretory vessels.  The present form differs from Stilesia hepatica, Wolffhugel (1903) [3],in having the mature segments broader than long, craspedote, testes in two groups,6-7 on each side, pre ovarian, in anterior half of the segments; vas deferens not closely coiled, but extends up to longitudinal excretory vessels; cirrus pouch almost oval, ovary small, compact, oval; vagina anterior to the cirrus pouch, uterus a transverse and small tube, in the centre; par uterine organ situated internal to the longitudinal excretory vessel.  The present tapeworm differs from Stilesia leiperi Kadam et.al.,(1980) [4], which is having the mature segments broader than long, testes in two groups,5-6 on each side, postero lateral to ovary; vas deferens not closely coiled, but extends beyond the longitudinal excretory canals; cirrus pouch elongated, cylindrical; ovary medium, almost circular, compact, with small acini; vagina posterior to cirrus pouch, uterus a broader transverse tube, in the anterior half of the segment, with two lateral sacs, having par uterine organs, at distal end and develops from uterus, situated in between dorsal and ventral excretory canals.  The present worm, differs from Stilesia caballeroi Kalyankar et.al.,(1981) [5], in having the testes 1-11 on each side, vas deferens forms a less dense bundle of convolutions, anterior to the testes; cirrus pouch oval, vagina opens into the genital cloaca, posterior to the cirrus pouch and the par uterine organs spherical on postero lateral surface.  The present cestode differs from Stilesia southwelli Shinde et.al.,(1982) [6], in having mature segments much broader than long, in two groups; vas deferens very much coiled, reaching up to ovary; cirrus pouch large, sac like; ovary medium, almost round, compact, without acini, in the centre of the segments; vagina posterior to the cirrus pouch, uterus secular, a thin transverse tube and two par uterine organs in each gravid segment, which are large, oval, containing 10-15 eggs.  The present tapeworm differs from Stilesia aurangabadensis, Majjid et.al.,(1982) [7], in having mature segments much more broader than long; testes in two lateral groups, round 5 on each side; vas deferens straight reaches to the longitudinal excretory canals; cirrus pouch elongated, cylindrical; cirrus coiled; ovary medium, circular compact; vagina a thin tube and postero dorsal to cirrus pouch.  The present worm differs from Stilesia garhwalensis Malhotra and Capoor (1983) [8], in having mature segments broader than long, testes 0-9 in number, on each side, oval to spherical; cirrus pouch oval, extending half way between lateral margin and longitudinal excretory canal; ovary small, spherical, vas deferens coiled, par uterine organs two in number, one on each lateral side of the segment.  The present tapeworm, differs from Stilesia kotdwarensis, Malhotra and Capoor (1983) [8], in having the mature segment broader than long, testes oval to spherical,1-2 in number, on each side, the number varies from segment to segment, maximum 6 on each side; vas deferens coiled, reaches up to the longitudinal excretory canals; cirrus pouch oval, extending half across between lateral margins and ventral to longitudinal excretory canal; ovary small, spherical; vagina posterior to the cirrus pouch; par uterine organs small, between dorsal and ventral longitudinal excretory canals, two in each gravid segment.  The present form differs from Stilesia marathwadaensis Shinde et.al., (1985) [9], in having the mature segments broader than long, testes round, in two groups, 5-7 in number, postero lateral to ovary; vas deferens not coiled, cirrus pouch oval, elongated, cylindrical, present at the anterior margin of segment; ovary medium in size, oval in shape, compact in anterior half of the segments; vagina on the posterior side of the cirrus pouch, par uterine organs two in number, oval in shape, large in size.  The present worm differs from Stilesia jadhavae, Jadhav (1999) [10], in having large ovary as compared to the present worm.  The worm under discussion differs from Stilesia yavalensis Shinde and Kalse (1999) [11], which has quadrangular scolex and from Stilesia dhondagae Deshmukh et.al., (2001) in having quadrangular scolex and testes 8-10 in number, arranged in two rows, vas deferens short.
 The present worm differs from Stilesia capari Patil and Menkudle (2002) [12], in having vas deferens short and ovary sac like.  The present form differs from Stilesia pandeyi Nanware et al., (2004) [13], in number of testes and shape of ovary.  The present cestode differs from Stilesia indapurensis Khadap (2004) [14], in having vas deferens curved; It also differs from Stilesia daulatabadensis Shelke and Shinde (2004) in having acraspedote testes and vas deferens slightly curved.  The present cestode parasite differs from Stilasia ambajogaensis Pawar et.al. (2004) [15], in having mature segments broader than long; testes 25-30 (28) medium, oval, arranged in three fields, in two lateral groups in the central medulla, third group having three testes in each segment, outside the longitudinal excretory canals, vas deferens short, thin tube; cirrus pouch medium long; ovary small, oval, a single mass; vagina thin, medium, curved, posterior to the cirrus pouch; genital pore small, oval, regularly alternate.  It also differs from Stilesia jadhavi Nanware and Jadhav (2005) [16], in having testes 14 in number and ovary bilobed.  The present worm differs from Stilesia govindae Padwal and Jadhav (2006) [17], which has ovary lobulated and testes12-14 in number and also from Stilesia kanegaonensis Patil (2012) in having vas deferens short and ovary divided into two pairs.  The present cestode differs from Stilesia shindei Lakhe (2018) [18], in having the mature segments squarish, broader than long, almost 16 times broader than long; testes medium, oval, 11-14 in number, 5+6 or 6+8 in each segment; ovary medium, oval, single mass, compact. : The name of the species is proposed after the author's father surname.

Conclusion
The above noted characters are valid enough to erect a new species Stilesia jadhavae sp.nov. Hence the name of the species is proposed after the author's father surname.